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KMID : 0613820110210050626
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Journal of Life Science
2011 Volume.21 No. 5 p.626 ~ p.630
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Sex Identification of the First Incubated Chicks of the Crested Ibis Nipponia nippon in Korea
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Kim Kyung-A
Cha Jae-Seok
Kim Tae-Jwa
Kim Jyung-min
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Abstract
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In October 2008, a pair of Crested ibis Nipponia nippon, an endangered avian species in the world, was donated to Korea from China. They have since been the subject of a successful program to incubate chicks for the first time in South Korea. This study was carried out to determine the sex of chicks from the Crested ibis through polymerase chain reaction (PCR) using the sex-related gene and the chromodomain helicase DNA binding protein (CHD) gene. The result of the CHD gene, which was used with a single set of primers and a restriction enzyme treatment after the PCR process, was more accurate in identifying the gender of the Crested ibis. In addition, we compared the CHD gene sequences with the previously reported sequences and found 1~2 different bases between females (CI2, CI4, CI5, and CI6) than in studies previously reporting female sequences.
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KEYWORD
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Sex identification, crested ibis, Nipponia nippon, sex-related gene, CHD gene
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